Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv

Fluorescent labeling of proteins is a widespread approach for the microscopic examination of protein function, expression, and localization in the cell. Here, we present a protocol for the labeling of hemagglutinin (HA)-tagged protein of interest (POI) with the single-chain antibody (scFv) 2E2 fused...

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Detalles Bibliográficos
Autores principales: Tsirkas, Ioannis, Zur, Tomer, Dovrat, Daniel, Paleiov, Zohar, Ravkaie, Lior, Aharoni, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251152/
https://www.ncbi.nlm.nih.gov/pubmed/37270779
http://dx.doi.org/10.1016/j.xpro.2023.102299

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251152/
https://www.ncbi.nlm.nih.gov/pubmed/37270779
http://dx.doi.org/10.1016/j.xpro.2023.102299

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