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Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge
SIMPLE SUMMARY: As a consequence of the increased use of plant ingredients in marine fish diets, there is a trend for a reduction in long-chain polyunsaturated fatty acid content and an increase in carbohydrates in aquafeeds. Thus, it is important to understand the ways in which such dietary modific...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251966/ https://www.ncbi.nlm.nih.gov/pubmed/37889635 http://dx.doi.org/10.3390/ani13111770 |
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author | Magalhães, Rui Martins, Nicole Fontinha, Filipa Olsen, Rolf Erick Serra, Claudia Reis Peres, Helena Oliva-Teles, Aires |
author_facet | Magalhães, Rui Martins, Nicole Fontinha, Filipa Olsen, Rolf Erick Serra, Claudia Reis Peres, Helena Oliva-Teles, Aires |
author_sort | Magalhães, Rui |
collection | PubMed |
description | SIMPLE SUMMARY: As a consequence of the increased use of plant ingredients in marine fish diets, there is a trend for a reduction in long-chain polyunsaturated fatty acid content and an increase in carbohydrates in aquafeeds. Thus, it is important to understand the ways in which such dietary modifications impact the health status and disease resistance of fish. Considering this, we evaluated the effects of high dietary inclusions of arachidonic acid (ARA) or docosahexaenoic acid (DHA) together with high or low inclusion of digestible carbohydrates (CHO) before and after a bacterial challenge with killed Photobacterium damselae subsp. piscicida (Phdp) on selected immune parameters. Our results showed that high dietary ARA levels improved the fish immune response to a bacterial challenge. The dietary CHO provides important energy to promote a fast response of the gilthead sea bream immune system. ABSTRACT: This study aims to assess the effects of different dietary n-6/n-3 long-chain polyunsaturated fatty acid ratios and CHO content in the immune response of gilthead seabream. For that purpose, gilthead sea bream juveniles (initial body weight = 47.5 g) were fed for 84 days with four isoproteic (47% crude protein) and isolipidic (18% crude lipids) diets with high (20%) or low (5%) level of gelatinized starch (HS or LS diets, respectively) and included approximately 2.4% ARA or DHA. At the end of the trial, the DHA-enriched groups presented increased red blood cell (RBC) count, hemoglobin, plasmatic nitric oxide (NO) content, and antiprotease and alternative complement activities. The ARA groups had increased thrombocyte count, and plasmatic bactericidal activity against Vibrio anguillarum was lower in the fish fed the ARA/LS diet. After the feeding trial, the fish were challenged with an intraperitoneal injection (i.p.) of killed Photobacterium damselae subsp. piscicida (Phdp) and sampled at 4 and 24 h after the challenge. At 4 h after i.p., the ARA groups presented increased plasma total immunoglobulins (Ig) and bactericidal activity against V. anguillarum. In addition, the fish fed the ARA/LS diet presented lower white blood cell (WBC) and alternative complement activity. At 24 h after i.p., the ARA groups presented increased RBC, WBC, and thrombocyte numbers, total IG, plasma peroxidase activity, and casp3 expression in the distal intestine. The HS groups presented increased plasma NO content and bactericidal activity against Phdp and decreased protease, antiprotease activity, and bactericidal activity against V. anguillarum. In conclusion, high dietary DHA levels seemed to improve the immune status of unchallenged gilthead sea bream juveniles, while high dietary ARA levels improved the fish immune response to a bacterial challenge. The energy provided by dietary starch seems to be important to promote a fast response by the fish immune system after a challenge. |
format | Online Article Text |
id | pubmed-10251966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102519662023-06-10 Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge Magalhães, Rui Martins, Nicole Fontinha, Filipa Olsen, Rolf Erick Serra, Claudia Reis Peres, Helena Oliva-Teles, Aires Animals (Basel) Article SIMPLE SUMMARY: As a consequence of the increased use of plant ingredients in marine fish diets, there is a trend for a reduction in long-chain polyunsaturated fatty acid content and an increase in carbohydrates in aquafeeds. Thus, it is important to understand the ways in which such dietary modifications impact the health status and disease resistance of fish. Considering this, we evaluated the effects of high dietary inclusions of arachidonic acid (ARA) or docosahexaenoic acid (DHA) together with high or low inclusion of digestible carbohydrates (CHO) before and after a bacterial challenge with killed Photobacterium damselae subsp. piscicida (Phdp) on selected immune parameters. Our results showed that high dietary ARA levels improved the fish immune response to a bacterial challenge. The dietary CHO provides important energy to promote a fast response of the gilthead sea bream immune system. ABSTRACT: This study aims to assess the effects of different dietary n-6/n-3 long-chain polyunsaturated fatty acid ratios and CHO content in the immune response of gilthead seabream. For that purpose, gilthead sea bream juveniles (initial body weight = 47.5 g) were fed for 84 days with four isoproteic (47% crude protein) and isolipidic (18% crude lipids) diets with high (20%) or low (5%) level of gelatinized starch (HS or LS diets, respectively) and included approximately 2.4% ARA or DHA. At the end of the trial, the DHA-enriched groups presented increased red blood cell (RBC) count, hemoglobin, plasmatic nitric oxide (NO) content, and antiprotease and alternative complement activities. The ARA groups had increased thrombocyte count, and plasmatic bactericidal activity against Vibrio anguillarum was lower in the fish fed the ARA/LS diet. After the feeding trial, the fish were challenged with an intraperitoneal injection (i.p.) of killed Photobacterium damselae subsp. piscicida (Phdp) and sampled at 4 and 24 h after the challenge. At 4 h after i.p., the ARA groups presented increased plasma total immunoglobulins (Ig) and bactericidal activity against V. anguillarum. In addition, the fish fed the ARA/LS diet presented lower white blood cell (WBC) and alternative complement activity. At 24 h after i.p., the ARA groups presented increased RBC, WBC, and thrombocyte numbers, total IG, plasma peroxidase activity, and casp3 expression in the distal intestine. The HS groups presented increased plasma NO content and bactericidal activity against Phdp and decreased protease, antiprotease activity, and bactericidal activity against V. anguillarum. In conclusion, high dietary DHA levels seemed to improve the immune status of unchallenged gilthead sea bream juveniles, while high dietary ARA levels improved the fish immune response to a bacterial challenge. The energy provided by dietary starch seems to be important to promote a fast response by the fish immune system after a challenge. MDPI 2023-05-26 /pmc/articles/PMC10251966/ /pubmed/37889635 http://dx.doi.org/10.3390/ani13111770 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Magalhães, Rui Martins, Nicole Fontinha, Filipa Olsen, Rolf Erick Serra, Claudia Reis Peres, Helena Oliva-Teles, Aires Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title | Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title_full | Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title_fullStr | Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title_full_unstemmed | Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title_short | Dietary ARA, DHA, and Carbohydrate Ratios Affect the Immune Status of Gilthead Sea Bream Juveniles upon Bacterial Challenge |
title_sort | dietary ara, dha, and carbohydrate ratios affect the immune status of gilthead sea bream juveniles upon bacterial challenge |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251966/ https://www.ncbi.nlm.nih.gov/pubmed/37889635 http://dx.doi.org/10.3390/ani13111770 |
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