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Properties of Bovine Collagen as Influenced by High-Pressure Processing

The physical properties and structure of collagen treated with high-pressure technologies have not yet been investigated in detail. The main goal of this work was to determine whether this modern gentle technology significantly changes the properties of collagen. High pressure in the range of 0–400...

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Autores principales: Houška, Milan, Landfeld, Aleš, Novotná, Pavla, Strohalm, Jan, Šupová, Monika, Suchý, Tomáš, Chlup, Hynek, Skočilas, Jan, Štípek, Jan, Žaloudková, Margit, Šulc, Miloslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255401/
https://www.ncbi.nlm.nih.gov/pubmed/37299271
http://dx.doi.org/10.3390/polym15112472
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author Houška, Milan
Landfeld, Aleš
Novotná, Pavla
Strohalm, Jan
Šupová, Monika
Suchý, Tomáš
Chlup, Hynek
Skočilas, Jan
Štípek, Jan
Žaloudková, Margit
Šulc, Miloslav
author_facet Houška, Milan
Landfeld, Aleš
Novotná, Pavla
Strohalm, Jan
Šupová, Monika
Suchý, Tomáš
Chlup, Hynek
Skočilas, Jan
Štípek, Jan
Žaloudková, Margit
Šulc, Miloslav
author_sort Houška, Milan
collection PubMed
description The physical properties and structure of collagen treated with high-pressure technologies have not yet been investigated in detail. The main goal of this work was to determine whether this modern gentle technology significantly changes the properties of collagen. High pressure in the range of 0–400 MPa was used, and the rheological, mechanical, thermal, and structural properties of collagen were measured. The rheological properties measured in the area of linear viscoelasticity do not statistically significantly change due to the influence of pressure or the duration of pressure exposure. In addition, the mechanical properties measured by compression between two plates are not statistically significantly influenced by pressure value or pressure hold time. The thermal properties T(on) and ∆H measured by differential calorimetry depend on pressure value and pressure hold time. Results from amino acids and FTIR analyses show that exposure of collagenous gels to high pressure (400 MPa), regardless of applied time (5 and 10 min), caused only minor changes in the primary and secondary structure and preserved collagenous polymeric integrity. SEM analysis did not show changes in collagen fibril ordering orientation over longer distances after applying 400 MPa of pressure for 10 min.
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spelling pubmed-102554012023-06-10 Properties of Bovine Collagen as Influenced by High-Pressure Processing Houška, Milan Landfeld, Aleš Novotná, Pavla Strohalm, Jan Šupová, Monika Suchý, Tomáš Chlup, Hynek Skočilas, Jan Štípek, Jan Žaloudková, Margit Šulc, Miloslav Polymers (Basel) Article The physical properties and structure of collagen treated with high-pressure technologies have not yet been investigated in detail. The main goal of this work was to determine whether this modern gentle technology significantly changes the properties of collagen. High pressure in the range of 0–400 MPa was used, and the rheological, mechanical, thermal, and structural properties of collagen were measured. The rheological properties measured in the area of linear viscoelasticity do not statistically significantly change due to the influence of pressure or the duration of pressure exposure. In addition, the mechanical properties measured by compression between two plates are not statistically significantly influenced by pressure value or pressure hold time. The thermal properties T(on) and ∆H measured by differential calorimetry depend on pressure value and pressure hold time. Results from amino acids and FTIR analyses show that exposure of collagenous gels to high pressure (400 MPa), regardless of applied time (5 and 10 min), caused only minor changes in the primary and secondary structure and preserved collagenous polymeric integrity. SEM analysis did not show changes in collagen fibril ordering orientation over longer distances after applying 400 MPa of pressure for 10 min. MDPI 2023-05-26 /pmc/articles/PMC10255401/ /pubmed/37299271 http://dx.doi.org/10.3390/polym15112472 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Houška, Milan
Landfeld, Aleš
Novotná, Pavla
Strohalm, Jan
Šupová, Monika
Suchý, Tomáš
Chlup, Hynek
Skočilas, Jan
Štípek, Jan
Žaloudková, Margit
Šulc, Miloslav
Properties of Bovine Collagen as Influenced by High-Pressure Processing
title Properties of Bovine Collagen as Influenced by High-Pressure Processing
title_full Properties of Bovine Collagen as Influenced by High-Pressure Processing
title_fullStr Properties of Bovine Collagen as Influenced by High-Pressure Processing
title_full_unstemmed Properties of Bovine Collagen as Influenced by High-Pressure Processing
title_short Properties of Bovine Collagen as Influenced by High-Pressure Processing
title_sort properties of bovine collagen as influenced by high-pressure processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255401/
https://www.ncbi.nlm.nih.gov/pubmed/37299271
http://dx.doi.org/10.3390/polym15112472
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