Cargando…
Co-translational binding of importins to nascent proteins
Various cellular quality control mechanisms support proteostasis. While, ribosome-associated chaperones prevent the misfolding of nascent chains during translation, importins were shown to prevent the aggregation of specific cargoes in a post-translational mechanism prior the import into the nucleop...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256725/ https://www.ncbi.nlm.nih.gov/pubmed/37296145 http://dx.doi.org/10.1038/s41467-023-39150-9 |
_version_ | 1785057167843262464 |
---|---|
author | Seidel, Maximilian Romanov, Natalie Obarska-Kosinska, Agnieszka Becker, Anja Trevisan Doimo de Azevedo, Nayara Provaznik, Jan Nagaraja, Sankarshana R. Landry, Jonathan J. M. Benes, Vladimir Beck, Martin |
author_facet | Seidel, Maximilian Romanov, Natalie Obarska-Kosinska, Agnieszka Becker, Anja Trevisan Doimo de Azevedo, Nayara Provaznik, Jan Nagaraja, Sankarshana R. Landry, Jonathan J. M. Benes, Vladimir Beck, Martin |
author_sort | Seidel, Maximilian |
collection | PubMed |
description | Various cellular quality control mechanisms support proteostasis. While, ribosome-associated chaperones prevent the misfolding of nascent chains during translation, importins were shown to prevent the aggregation of specific cargoes in a post-translational mechanism prior the import into the nucleoplasm. Here, we hypothesize that importins may already bind ribosome-associated cargo in a co-translational manner. We systematically measure the nascent chain association of all importins in Saccharomyces cerevisiae by selective ribosome profiling. We identify a subset of importins that bind to a wide range of nascent, often uncharacterized cargoes. This includes ribosomal proteins, chromatin remodelers and RNA binding proteins that are aggregation prone in the cytosol. We show that importins act consecutively with other ribosome-associated chaperones. Thus, the nuclear import system is directly intertwined with nascent chain folding and chaperoning. |
format | Online Article Text |
id | pubmed-10256725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102567252023-06-11 Co-translational binding of importins to nascent proteins Seidel, Maximilian Romanov, Natalie Obarska-Kosinska, Agnieszka Becker, Anja Trevisan Doimo de Azevedo, Nayara Provaznik, Jan Nagaraja, Sankarshana R. Landry, Jonathan J. M. Benes, Vladimir Beck, Martin Nat Commun Article Various cellular quality control mechanisms support proteostasis. While, ribosome-associated chaperones prevent the misfolding of nascent chains during translation, importins were shown to prevent the aggregation of specific cargoes in a post-translational mechanism prior the import into the nucleoplasm. Here, we hypothesize that importins may already bind ribosome-associated cargo in a co-translational manner. We systematically measure the nascent chain association of all importins in Saccharomyces cerevisiae by selective ribosome profiling. We identify a subset of importins that bind to a wide range of nascent, often uncharacterized cargoes. This includes ribosomal proteins, chromatin remodelers and RNA binding proteins that are aggregation prone in the cytosol. We show that importins act consecutively with other ribosome-associated chaperones. Thus, the nuclear import system is directly intertwined with nascent chain folding and chaperoning. Nature Publishing Group UK 2023-06-09 /pmc/articles/PMC10256725/ /pubmed/37296145 http://dx.doi.org/10.1038/s41467-023-39150-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Seidel, Maximilian Romanov, Natalie Obarska-Kosinska, Agnieszka Becker, Anja Trevisan Doimo de Azevedo, Nayara Provaznik, Jan Nagaraja, Sankarshana R. Landry, Jonathan J. M. Benes, Vladimir Beck, Martin Co-translational binding of importins to nascent proteins |
title | Co-translational binding of importins to nascent proteins |
title_full | Co-translational binding of importins to nascent proteins |
title_fullStr | Co-translational binding of importins to nascent proteins |
title_full_unstemmed | Co-translational binding of importins to nascent proteins |
title_short | Co-translational binding of importins to nascent proteins |
title_sort | co-translational binding of importins to nascent proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256725/ https://www.ncbi.nlm.nih.gov/pubmed/37296145 http://dx.doi.org/10.1038/s41467-023-39150-9 |
work_keys_str_mv | AT seidelmaximilian cotranslationalbindingofimportinstonascentproteins AT romanovnatalie cotranslationalbindingofimportinstonascentproteins AT obarskakosinskaagnieszka cotranslationalbindingofimportinstonascentproteins AT beckeranja cotranslationalbindingofimportinstonascentproteins AT trevisandoimodeazevedonayara cotranslationalbindingofimportinstonascentproteins AT provaznikjan cotranslationalbindingofimportinstonascentproteins AT nagarajasankarshanar cotranslationalbindingofimportinstonascentproteins AT landryjonathanjm cotranslationalbindingofimportinstonascentproteins AT benesvladimir cotranslationalbindingofimportinstonascentproteins AT beckmartin cotranslationalbindingofimportinstonascentproteins |