Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity

Genome editing with CRISPR-associated (Cas) proteins holds exceptional promise for “correcting” variants causing genetic disease. To realize this promise, off-target genomic changes cannot occur during the editing process. Here, we use whole genome sequencing to compare the genomes of 50 Cas9-edited...

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Detalles Bibliográficos
Autores principales: Peterson, Kevin A., Khalouei, Sam, Hanafi, Nour, Wood, Joshua A., Lanza, Denise G., Lintott, Lauri G., Willis, Brandon J., Seavitt, John R., Braun, Robert E., Dickinson, Mary E., White, Jacqueline K., Lloyd, K. C. Kent, Heaney, Jason D., Murray, Stephen A., Ramani, Arun, Nutter, Lauryl M. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257658/
https://www.ncbi.nlm.nih.gov/pubmed/37301944
http://dx.doi.org/10.1038/s42003-023-04974-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257658/
https://www.ncbi.nlm.nih.gov/pubmed/37301944
http://dx.doi.org/10.1038/s42003-023-04974-0

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