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Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity
Genome editing with CRISPR-associated (Cas) proteins holds exceptional promise for “correcting” variants causing genetic disease. To realize this promise, off-target genomic changes cannot occur during the editing process. Here, we use whole genome sequencing to compare the genomes of 50 Cas9-edited...
Autores principales: | Peterson, Kevin A., Khalouei, Sam, Hanafi, Nour, Wood, Joshua A., Lanza, Denise G., Lintott, Lauri G., Willis, Brandon J., Seavitt, John R., Braun, Robert E., Dickinson, Mary E., White, Jacqueline K., Lloyd, K. C. Kent, Heaney, Jason D., Murray, Stephen A., Ramani, Arun, Nutter, Lauryl M. J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257658/ https://www.ncbi.nlm.nih.gov/pubmed/37301944 http://dx.doi.org/10.1038/s42003-023-04974-0 |
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