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Balancing Nonsense Mutation Readthrough and Toxicity of Designer Aminoglycosides for Treatment of Genetic Diseases

[Image: see text] New derivatives of aminoglycosides with a side chain 1,2-aminoalcohol at the 5” position of ring III were designed, synthesized, and biologically evaluated. The novel lead structure (compound 6), exhibiting substantially enhanced selectivity toward eukaryotic versus prokaryotic rib...

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Detalles Bibliográficos
Autores principales: Guchhait, Sandip, Khononov, Alina, Pieńko, Tomasz, Belakhov, Valery, Baasov, Timor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258827/
https://www.ncbi.nlm.nih.gov/pubmed/37312846
http://dx.doi.org/10.1021/acsmedchemlett.3c00089
Descripción
Sumario:[Image: see text] New derivatives of aminoglycosides with a side chain 1,2-aminoalcohol at the 5” position of ring III were designed, synthesized, and biologically evaluated. The novel lead structure (compound 6), exhibiting substantially enhanced selectivity toward eukaryotic versus prokaryotic ribosome, high readthrough activity, and considerably lower toxicity than the previous lead compounds, was discovered. Balanced readthrough activity and toxicity of 6 were demonstrated in three different nonsense DNA-constructs underlying the genetic diseases, cystic fibrosis and Usher syndrome, and in two different cell lines, baby hamster kidney and human embryonic kidney cells. Molecular dynamics simulations within the A site of the 80S yeast ribosome demonstrated a remarkable kinetic stability of 6, which potentially determines its high readthrough activity.