Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast

A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and multi-site genome editing approach that combines all edits without selection markers using CRISPR-Cas9....

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Detalles Bibliográficos
Autores principales: Abdullah, Mudabir, Greco, Brittany M., Laurent, Jon M., Garge, Riddhiman K., Boutz, Daniel R., Vandeloo, Michelle, Marcotte, Edward M., Kachroo, Aashiq H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261898/
https://www.ncbi.nlm.nih.gov/pubmed/37323580
http://dx.doi.org/10.1016/j.crmeth.2023.100464

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261898/
https://www.ncbi.nlm.nih.gov/pubmed/37323580
http://dx.doi.org/10.1016/j.crmeth.2023.100464

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