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Revised iCLIP-seq Protocol for Profiling RNA–protein Interaction Sites at Individual Nucleotide Resolution in Living Cells
Individual nucleotide resolution UV cross-linking and immunoprecipitation followed by high-throughput sequencing (iCLIP-seq) is a powerful technique that is used to identify RNA-binding proteins’ (RBP) binding sites on target RNAs and to characterize the molecular basis of posttranscriptional regula...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Bio-Protocol
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262072/ https://www.ncbi.nlm.nih.gov/pubmed/37323634 http://dx.doi.org/10.21769/BioProtoc.4688 |