Cycloheximide (CHX) Chase Assay to Examine Protein Half-life

Cycloheximide (CHX) is a small molecule derived from Streptomyces griseus that acts as fungicide. As a ribosome inhibitor, CHX can restrict the translation elongation of eukaryotic protein synthesis. Once protein synthesis is inhibited by CHX, the level of intracellular proteins decreases by degrada...

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Detalles Bibliográficos
Autores principales: Miao, Ying, Du, Qian, Zhang, Hong-Guang, Yuan, Yukang, Zuo, Yibo, Zheng, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262206/
https://www.ncbi.nlm.nih.gov/pubmed/37323633
http://dx.doi.org/10.21769/BioProtoc.4690

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262206/
https://www.ncbi.nlm.nih.gov/pubmed/37323633
http://dx.doi.org/10.21769/BioProtoc.4690

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