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CRISPR activation and interference as investigative tools in the cardiovascular system

CRISPR activation and interference (CRISPRa/i) technology offers the unprecedented possibility of achieving regulated gene expression both in vitro and in vivo. The DNA pairing specificity of a nuclease dead Cas9 (dCas9) is exploited to precisely target a transcriptional activator or repressor in pr...

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Detalles Bibliográficos
Autores principales: Carroll, Melissa S., Giacca, Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265131/
https://www.ncbi.nlm.nih.gov/pubmed/36563996
http://dx.doi.org/10.1016/j.biocel.2022.106348
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author Carroll, Melissa S.
Giacca, Mauro
author_facet Carroll, Melissa S.
Giacca, Mauro
author_sort Carroll, Melissa S.
collection PubMed
description CRISPR activation and interference (CRISPRa/i) technology offers the unprecedented possibility of achieving regulated gene expression both in vitro and in vivo. The DNA pairing specificity of a nuclease dead Cas9 (dCas9) is exploited to precisely target a transcriptional activator or repressor in proximity to a gene promoter. This permits both the study of phenotypes arising from gene modulation for investigative purposes, and the development of potential therapeutics. As with virtually all other organ systems, the cardiovascular system can deeply benefit from a broader utilisation of CRISPRa/i. However, application of this technology is still in its infancy. Significant areas for improvement include the identification of novel and more effective transcriptional regulators that can be docked to dCas9, and the development of more efficient methods for their delivery and expression in vivo.
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spelling pubmed-102651312023-06-15 CRISPR activation and interference as investigative tools in the cardiovascular system Carroll, Melissa S. Giacca, Mauro Int J Biochem Cell Biol Article CRISPR activation and interference (CRISPRa/i) technology offers the unprecedented possibility of achieving regulated gene expression both in vitro and in vivo. The DNA pairing specificity of a nuclease dead Cas9 (dCas9) is exploited to precisely target a transcriptional activator or repressor in proximity to a gene promoter. This permits both the study of phenotypes arising from gene modulation for investigative purposes, and the development of potential therapeutics. As with virtually all other organ systems, the cardiovascular system can deeply benefit from a broader utilisation of CRISPRa/i. However, application of this technology is still in its infancy. Significant areas for improvement include the identification of novel and more effective transcriptional regulators that can be docked to dCas9, and the development of more efficient methods for their delivery and expression in vivo. Elsevier 2023-02 /pmc/articles/PMC10265131/ /pubmed/36563996 http://dx.doi.org/10.1016/j.biocel.2022.106348 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carroll, Melissa S.
Giacca, Mauro
CRISPR activation and interference as investigative tools in the cardiovascular system
title CRISPR activation and interference as investigative tools in the cardiovascular system
title_full CRISPR activation and interference as investigative tools in the cardiovascular system
title_fullStr CRISPR activation and interference as investigative tools in the cardiovascular system
title_full_unstemmed CRISPR activation and interference as investigative tools in the cardiovascular system
title_short CRISPR activation and interference as investigative tools in the cardiovascular system
title_sort crispr activation and interference as investigative tools in the cardiovascular system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265131/
https://www.ncbi.nlm.nih.gov/pubmed/36563996
http://dx.doi.org/10.1016/j.biocel.2022.106348
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