Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage

Radical S-adenosyl-l-methionine (SAM) enzymes are ubiquitous in nature and carry out a broad variety of difficult chemical transformations initiated by hydrogen atom abstraction. Although numerous radical SAM (RS) enzymes have been structurally characterized, many prove recalcitrant to crystallizati...

Descripción completa

Detalles Bibliográficos
Autores principales: Moody, James D., Hill, Sarah, Lundahl, Maike N., Saxton, Aubrianna J., Galambas, Amanda, Broderick, William E., Lawrence, C. Martin, Broderick, Joan B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267522/
https://www.ncbi.nlm.nih.gov/pubmed/37156396
http://dx.doi.org/10.1016/j.jbc.2023.104791

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267522/
https://www.ncbi.nlm.nih.gov/pubmed/37156396
http://dx.doi.org/10.1016/j.jbc.2023.104791

Ejemplares similares