A novel BRET-based assay to investigate binding and residence time of unmodified ligands to the human lysosomal ion channel TRPML1 in intact cells

Here, we report a bioluminescence resonance energy transfer (BRET) assay as a novel way to investigate the binding of unlabeled ligands to the human transient receptor potential mucolipin 1 (hTRPML1), a lysosomal ion channel involved in several genetic diseases and cancer progression. This novel BRE...

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Detalles Bibliográficos
Autores principales: Cunha, Micael R., Catta-Preta, Carolina M.C., Takarada, Jéssica E., Moreira, Gabriela A., Massirer, Katlin B., Couñago, Rafael M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267526/
https://www.ncbi.nlm.nih.gov/pubmed/37172730
http://dx.doi.org/10.1016/j.jbc.2023.104807

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267526/
https://www.ncbi.nlm.nih.gov/pubmed/37172730
http://dx.doi.org/10.1016/j.jbc.2023.104807

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