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Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2

Carryover contamination during amplicon sequencing workflow (AMP-Seq) put the accuracy of the high-throughput detection for pathogens at risk. The purpose of this study is to develop a carryover contaminations-controlled AMP-Seq (ccAMP-Seq) workflow to enable accurate qualitative and quantitative de...

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Detalles Bibliográficos
Autores principales: Gao, Lifen, Li, Lun, Fang, Bin, Fang, Zhiwei, Xiang, Yanghai, Zhang, Min, Zhou, Junfei, Song, Huiyin, Chen, Lihong, Li, Tiantian, Xiao, Huafeng, Wan, Renjing, Jiang, Yongzhong, Peng, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269707/
https://www.ncbi.nlm.nih.gov/pubmed/37098913
http://dx.doi.org/10.1128/spectrum.00206-23