A ligation-independent sequencing method reveals tRNA-derived RNAs with blocked 3’ termini

Despite the numerous sequencing methods available, the vast diversity in size and chemical modifications of RNA molecules makes the capture of the full spectrum of cellular RNAs a difficult task. By combining quasi-random hexamer priming with a custom template switching strategy, we developed a meth...

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Detalles Bibliográficos
Autores principales: Scacchetti, Alessandro, Shields, Emily J., Trigg, Natalie A., Wilusz, Jeremy E., Conine, Colin C., Bonasio, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274639/
https://www.ncbi.nlm.nih.gov/pubmed/37333231
http://dx.doi.org/10.1101/2023.06.06.543899

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274639/
https://www.ncbi.nlm.nih.gov/pubmed/37333231
http://dx.doi.org/10.1101/2023.06.06.543899

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