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A ligation-independent sequencing method reveals tRNA-derived RNAs with blocked 3’ termini
Despite the numerous sequencing methods available, the vast diversity in size and chemical modifications of RNA molecules makes the capture of the full spectrum of cellular RNAs a difficult task. By combining quasi-random hexamer priming with a custom template switching strategy, we developed a meth...
Autores principales: | Scacchetti, Alessandro, Shields, Emily J., Trigg, Natalie A., Wilusz, Jeremy E., Conine, Colin C., Bonasio, Roberto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274639/ https://www.ncbi.nlm.nih.gov/pubmed/37333231 http://dx.doi.org/10.1101/2023.06.06.543899 |
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