Artifacts and biases of the reverse transcription reaction in RNA sequencing

RNA sequencing has spurred a significant number of research areas in recent years. Most protocols rely on synthesizing a more stable complementary DNA (cDNA) copy of the RNA molecule during the reverse transcription reaction. The resulting cDNA pool is often wrongfully assumed to be quantitatively a...

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Detalles Bibliográficos
Autores principales: Verwilt, Jasper, Mestdagh, Pieter, Vandesompele, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Mini-Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275267/
https://www.ncbi.nlm.nih.gov/pubmed/36990512
http://dx.doi.org/10.1261/rna.079623.123

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275267/
https://www.ncbi.nlm.nih.gov/pubmed/36990512
http://dx.doi.org/10.1261/rna.079623.123

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