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Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain

AIMS: : To determine whether the blood–brain barrier (BBB) opens to enhance drug delivery during the acute stage of unsaturated fat embolism. METHODS: : We infused oleic, linoleic, and linolenic acid emulsions through the right common carotid artery of rats, followed by trypan blue for gross and lan...

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Autores principales: Kim, Yong‐Woo, Choi, Seon Hee, Choi, Ki Joo, Kim, Hak Jin, Kang, Jin Sook, Hong, Dong Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275518/
https://www.ncbi.nlm.nih.gov/pubmed/37218399
http://dx.doi.org/10.1002/brb3.2994
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author Kim, Yong‐Woo
Choi, Seon Hee
Choi, Ki Joo
Kim, Hak Jin
Kang, Jin Sook
Hong, Dong Jin
author_facet Kim, Yong‐Woo
Choi, Seon Hee
Choi, Ki Joo
Kim, Hak Jin
Kang, Jin Sook
Hong, Dong Jin
author_sort Kim, Yong‐Woo
collection PubMed
description AIMS: : To determine whether the blood–brain barrier (BBB) opens to enhance drug delivery during the acute stage of unsaturated fat embolism. METHODS: : We infused oleic, linoleic, and linolenic acid emulsions through the right common carotid artery of rats, followed by trypan blue for gross and lanthanum for electron microscopic (EM) examination. Doxorubicin and temozolomide were also administered, and then the rats were euthanized at 30 min, 1 h, and 2 h. Trypan blue hue was analyzed to semiquantitatively measure BBB opening. Desorption electrospray ionization–mass spectrometry (DESI–MS) imaging was used to evaluate drug delivery. RESULTS: Trypan blue staining observed in each group 30 min after emulsion infusion increased at 1 h and decreased after 2 h in the oleic acid group. The linoleic and linolenic acid groups showed weak staining over time. The hue and trypan blue analysis results were corroborative. EM showed tight junction opening, whereas DESI–MS imaging showed increased doxorubicin and temozolomide signal intensities in ipsilateral hemispheres of all three groups. CONCLUSION: We demonstrated that oleic, linoleic, and linolenic acid emulsions opened the BBB, promoting drug delivery to the brain. Hue analysis and DESI–MS imaging are appropriate for analysis of doxorubicin and temozolomide concentrations in brain tissue.
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spelling pubmed-102755182023-06-17 Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain Kim, Yong‐Woo Choi, Seon Hee Choi, Ki Joo Kim, Hak Jin Kang, Jin Sook Hong, Dong Jin Brain Behav Original Articles AIMS: : To determine whether the blood–brain barrier (BBB) opens to enhance drug delivery during the acute stage of unsaturated fat embolism. METHODS: : We infused oleic, linoleic, and linolenic acid emulsions through the right common carotid artery of rats, followed by trypan blue for gross and lanthanum for electron microscopic (EM) examination. Doxorubicin and temozolomide were also administered, and then the rats were euthanized at 30 min, 1 h, and 2 h. Trypan blue hue was analyzed to semiquantitatively measure BBB opening. Desorption electrospray ionization–mass spectrometry (DESI–MS) imaging was used to evaluate drug delivery. RESULTS: Trypan blue staining observed in each group 30 min after emulsion infusion increased at 1 h and decreased after 2 h in the oleic acid group. The linoleic and linolenic acid groups showed weak staining over time. The hue and trypan blue analysis results were corroborative. EM showed tight junction opening, whereas DESI–MS imaging showed increased doxorubicin and temozolomide signal intensities in ipsilateral hemispheres of all three groups. CONCLUSION: We demonstrated that oleic, linoleic, and linolenic acid emulsions opened the BBB, promoting drug delivery to the brain. Hue analysis and DESI–MS imaging are appropriate for analysis of doxorubicin and temozolomide concentrations in brain tissue. John Wiley and Sons Inc. 2023-05-22 /pmc/articles/PMC10275518/ /pubmed/37218399 http://dx.doi.org/10.1002/brb3.2994 Text en © 2023 The Authors. Brain and Behavior published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Kim, Yong‐Woo
Choi, Seon Hee
Choi, Ki Joo
Kim, Hak Jin
Kang, Jin Sook
Hong, Dong Jin
Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title_full Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title_fullStr Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title_full_unstemmed Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title_short Unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
title_sort unsaturated free fatty acid emulsion infusion into carotid artery enhances drug delivery to the rat brain
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275518/
https://www.ncbi.nlm.nih.gov/pubmed/37218399
http://dx.doi.org/10.1002/brb3.2994
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