Protocol to measure protein-RNA binding using double filter-binding assays followed by phosphorimaging or high-throughput sequencing

Binding affinity quantitatively describes the strength of a molecular interaction and is reported by the equilibrium dissociation constant (K(D)). Here, we present a protocol to measure K(D) of mammalian microRNA-loaded Argonaute2 protein by double filter binding. We describe steps for radiolabeling...

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Detalles Bibliográficos
Autores principales: Vega-Badillo, Joel, Zamore, Phillip D., Jouravleva, Karina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276142/
https://www.ncbi.nlm.nih.gov/pubmed/37270783
http://dx.doi.org/10.1016/j.xpro.2023.102336

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276142/
https://www.ncbi.nlm.nih.gov/pubmed/37270783
http://dx.doi.org/10.1016/j.xpro.2023.102336

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