Cargando…

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive. In this protocol, we adapt a previously designed FISH amplificatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Warren, Rachel, Shaik, Aftab, Teubner, Lauren, Lyu, Handeng, De Langhe, Stijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276273/
https://www.ncbi.nlm.nih.gov/pubmed/37302070
http://dx.doi.org/10.1016/j.xpro.2023.102353