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Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue
Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive. In this protocol, we adapt a previously designed FISH amplificatio...
Autores principales: | Warren, Rachel, Shaik, Aftab, Teubner, Lauren, Lyu, Handeng, De Langhe, Stijn |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276273/ https://www.ncbi.nlm.nih.gov/pubmed/37302070 http://dx.doi.org/10.1016/j.xpro.2023.102353 |
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