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Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

PREMISE: We present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output. The goal is to help those interested in generating long‐read sequencing data determine which step...

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Detalles Bibliográficos
Autores principales: De La Cerda, Gisel Y., Landis, Jacob B., Eifler, Evan, Hernandez, Adriana I., Li, Fay‐Wei, Zhang, Jing, Tribble, Carrie M., Karimi, Nisa, Chan, Patricia, Givnish, Thomas, Strickler, Susan R., Specht, Chelsea D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278932/
https://www.ncbi.nlm.nih.gov/pubmed/37342170
http://dx.doi.org/10.1002/aps3.11524