Testing protocols to optimize DNA extraction from tough leaf tissue: A case study in Encephalartos

PREMISE: Plants with stiff, leathery leaves pose a challenge for standard DNA extraction protocols. These tissues are recalcitrant to mechanical disruption via TissueLyser (or analogous devices) and are often high in secondary metabolites. These compounding factors result in low yields, which may be...

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Detalles Bibliográficos
Autores principales: Jones, Maia M., Nagalingum, Nathalie S., Handley, Vanessa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Protocol Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278938/
https://www.ncbi.nlm.nih.gov/pubmed/37342169
http://dx.doi.org/10.1002/aps3.11525

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278938/
https://www.ncbi.nlm.nih.gov/pubmed/37342169
http://dx.doi.org/10.1002/aps3.11525

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