High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells

CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To determine the specificity and the mutagenic spectrum of SpCas9 in tomato, we designed 89 g(uide) RNAs targeting genes of the tomato MYB transcription factor family with varying predicted specificities. Plasmids enco...

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Detalles Bibliográficos
Autores principales: Slaman, Ellen, Lammers, Michiel, Angenent, Gerco C., de Maagd, Ruud A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279869/
https://www.ncbi.nlm.nih.gov/pubmed/37346168
http://dx.doi.org/10.3389/fgeed.2023.1196763

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279869/
https://www.ncbi.nlm.nih.gov/pubmed/37346168
http://dx.doi.org/10.3389/fgeed.2023.1196763

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