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Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell r...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285093/ https://www.ncbi.nlm.nih.gov/pubmed/37360105 http://dx.doi.org/10.1016/j.heliyon.2023.e16792 |
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author | Ye, Qianyu Wang, Jiali Chen, Meijing Nie, Weijian Zhang, Huanxi Su, Xiaojun Ling, Liuting Liu, Xiangjun Liu, Longshan Wang, Changxi Gao, Yifang |
author_facet | Ye, Qianyu Wang, Jiali Chen, Meijing Nie, Weijian Zhang, Huanxi Su, Xiaojun Ling, Liuting Liu, Xiangjun Liu, Longshan Wang, Changxi Gao, Yifang |
author_sort | Ye, Qianyu |
collection | PubMed |
description | OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. METHODS: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. RESULTS: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. CONCLUSIONS: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications. |
format | Online Article Text |
id | pubmed-10285093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-102850932023-06-23 Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus Ye, Qianyu Wang, Jiali Chen, Meijing Nie, Weijian Zhang, Huanxi Su, Xiaojun Ling, Liuting Liu, Xiangjun Liu, Longshan Wang, Changxi Gao, Yifang Heliyon Research Article OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. METHODS: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. RESULTS: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. CONCLUSIONS: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications. Elsevier 2023-06-08 /pmc/articles/PMC10285093/ /pubmed/37360105 http://dx.doi.org/10.1016/j.heliyon.2023.e16792 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Ye, Qianyu Wang, Jiali Chen, Meijing Nie, Weijian Zhang, Huanxi Su, Xiaojun Ling, Liuting Liu, Xiangjun Liu, Longshan Wang, Changxi Gao, Yifang Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title_full | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title_fullStr | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title_full_unstemmed | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title_short | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
title_sort | interferon-gamma flowspot assay for the measurement of the t-cell response to cytomegalovirus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285093/ https://www.ncbi.nlm.nih.gov/pubmed/37360105 http://dx.doi.org/10.1016/j.heliyon.2023.e16792 |
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