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Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus

OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell r...

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Autores principales: Ye, Qianyu, Wang, Jiali, Chen, Meijing, Nie, Weijian, Zhang, Huanxi, Su, Xiaojun, Ling, Liuting, Liu, Xiangjun, Liu, Longshan, Wang, Changxi, Gao, Yifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285093/
https://www.ncbi.nlm.nih.gov/pubmed/37360105
http://dx.doi.org/10.1016/j.heliyon.2023.e16792
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author Ye, Qianyu
Wang, Jiali
Chen, Meijing
Nie, Weijian
Zhang, Huanxi
Su, Xiaojun
Ling, Liuting
Liu, Xiangjun
Liu, Longshan
Wang, Changxi
Gao, Yifang
author_facet Ye, Qianyu
Wang, Jiali
Chen, Meijing
Nie, Weijian
Zhang, Huanxi
Su, Xiaojun
Ling, Liuting
Liu, Xiangjun
Liu, Longshan
Wang, Changxi
Gao, Yifang
author_sort Ye, Qianyu
collection PubMed
description OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. METHODS: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. RESULTS: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. CONCLUSIONS: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications.
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spelling pubmed-102850932023-06-23 Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus Ye, Qianyu Wang, Jiali Chen, Meijing Nie, Weijian Zhang, Huanxi Su, Xiaojun Ling, Liuting Liu, Xiangjun Liu, Longshan Wang, Changxi Gao, Yifang Heliyon Research Article OBJECTIVES: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. METHODS: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. RESULTS: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. CONCLUSIONS: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications. Elsevier 2023-06-08 /pmc/articles/PMC10285093/ /pubmed/37360105 http://dx.doi.org/10.1016/j.heliyon.2023.e16792 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ye, Qianyu
Wang, Jiali
Chen, Meijing
Nie, Weijian
Zhang, Huanxi
Su, Xiaojun
Ling, Liuting
Liu, Xiangjun
Liu, Longshan
Wang, Changxi
Gao, Yifang
Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title_full Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title_fullStr Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title_full_unstemmed Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title_short Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
title_sort interferon-gamma flowspot assay for the measurement of the t-cell response to cytomegalovirus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285093/
https://www.ncbi.nlm.nih.gov/pubmed/37360105
http://dx.doi.org/10.1016/j.heliyon.2023.e16792
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