Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short reads

Deep parallel sequencing (NGS) is a viable tool for monitoring scFv and Fab library dynamics in many antibody engineering high-throughput screening efforts. Although very useful, the commonly used Illumina NGS platform cannot handle the entire sequence of scFv or Fab in a single read, usually focusi...

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Detalles Bibliográficos
Autores principales: Levin, Itay, Štrajbl, Marek, Fastman, Yair, Baran, Dror, Twito, Shir, Mioduser, Jessica, Keren, Adi, Fischman, Sharon, Zhenin, Michael, Nimrod, Guy, Levitin, Natalie, Mayor, May Ben, Gadrich, Meital, Ofran, Yanay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287906/
https://www.ncbi.nlm.nih.gov/pubmed/37014016
http://dx.doi.org/10.1093/nar/gkad235

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287906/
https://www.ncbi.nlm.nih.gov/pubmed/37014016
http://dx.doi.org/10.1093/nar/gkad235

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