Thermally controlled intein splicing of engineered DNA polymerases provides a robust and generalizable solution for accurate and sensitive molecular diagnostics

DNA polymerases are essential for nucleic acid synthesis, cloning, sequencing and molecular diagnostics technologies. Conditional intein splicing is a powerful tool for controlling enzyme reactions. We have engineered a thermal switch into thermostable DNA polymerases from two structurally distinct...

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Detalles Bibliográficos
Autores principales: Wang, You, Shi, Yuqian, Hellinga, Homme W, Beese, Lorena S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287962/
https://www.ncbi.nlm.nih.gov/pubmed/37166959
http://dx.doi.org/10.1093/nar/gkad368

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287962/
https://www.ncbi.nlm.nih.gov/pubmed/37166959
http://dx.doi.org/10.1093/nar/gkad368

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