Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans

In this study, an acidophilic GH5 β-mannanase (TaMan5) from Trichoderma asperellum ND-1 was efficiently expressed in Pichia pastoris (a 2.0-fold increase, 67.5 ± 1.95 U/mL). TaMan5 displayed the highest specificity toward locust bean gum (K(m) = 1.34 mg/mL, V(max) = 749.14 μmol/min/mg) at pH 4.0 and...

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Detalles Bibliográficos
Autores principales: Zheng, Fengzhen, Basit, Abdul, Wang, Jiaqiang, Zhuang, Huan, Chen, Jun, Zhang, Jianfen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288326/
https://www.ncbi.nlm.nih.gov/pubmed/37362936
http://dx.doi.org/10.3389/fmicb.2023.1191553

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288326/
https://www.ncbi.nlm.nih.gov/pubmed/37362936
http://dx.doi.org/10.3389/fmicb.2023.1191553

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