Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing

BACKGROUND: The CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research and hold great potential for future clinical applications. However, the short half-life of guide RNAs (gRNAs), particularly free gRNAs without Cas nuclease binding, limits their editing efficiency and du...

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Detalles Bibliográficos
Autores principales: Zhang, Xin, Wang, Xinlong, Lv, Jie, Huang, Hongxin, Wang, Jiahong, Zhuo, Ma, Tan, Zhihong, Huang, Guanjie, Liu, Jiawei, Liu, Yuchen, Li, Mengrao, Lin, Qixiao, Li, Lian, Ma, Shufeng, Huang, Tao, Lin, Ying, Zhao, Xiaoyang, Rong, Zhili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288759/
https://www.ncbi.nlm.nih.gov/pubmed/37353840
http://dx.doi.org/10.1186/s13059-023-02992-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288759/
https://www.ncbi.nlm.nih.gov/pubmed/37353840
http://dx.doi.org/10.1186/s13059-023-02992-z

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