Method versatility in RNA extraction-free PCR detection of SARS-CoV-2 in saliva samples

Early in the pandemic, a simple, open-source, RNA extraction-free RT-qPCR protocol for SARS-CoV-2 detection in saliva was developed and made widely available. This simplified approach (SalivaDirect) requires only sample treatment with proteinase K prior to PCR testing. However, feedback from clinica...

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Detalles Bibliográficos
Autores principales: Allicock, Orchid M., Yolda-Carr, Devyn, Earnest, Rebecca, Breban, Mallery I., Vega, Noel, Ott, Isabel M., Kalinich, Chaney, Alpert, Tara, Wyllie, Anne L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290768/
https://www.ncbi.nlm.nih.gov/pubmed/37369293
http://dx.doi.org/10.1016/j.pbiomolbio.2023.06.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290768/
https://www.ncbi.nlm.nih.gov/pubmed/37369293
http://dx.doi.org/10.1016/j.pbiomolbio.2023.06.004

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