The effect of low-abundance OTU filtering methods on the reliability and variability of microbial composition assessed by 16S rRNA amplicon sequencing

PCR amplicon sequencing may lead to detection of spurious operational taxonomic units (OTUs), inflating estimates of gut microbial diversity. There is no consensus in the analytical approach as to what filtering methods should be applied to remove low-abundance OTUs; moreover, few studies have inves...

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Detalles Bibliográficos
Autores principales: Nikodemova, Maria, Holzhausen, Elizabeth A., Deblois, Courtney L., Barnet, Jodi H., Peppard, Paul E., Suen, Garret, Malecki, Kristen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291178/
https://www.ncbi.nlm.nih.gov/pubmed/37377642
http://dx.doi.org/10.3389/fcimb.2023.1165295

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291178/
https://www.ncbi.nlm.nih.gov/pubmed/37377642
http://dx.doi.org/10.3389/fcimb.2023.1165295

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