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Lens regeneration in situ using hESCs-derived cells —similar to natural lens

Lens itself has limited regeneration functionality, thus we aimed to create regenerated lens with biological function to treat cataracts rather than the intraocular lens used in cataract surgery. We induced exogenous human embryonic stem cells to directionally differentiate into lens fate like cells...

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Autores principales: Chen, Xinyi, Wang, Hanle, Chen, Hui, Ren, Ling, Wang, Wei, Xu, Jingjie, Luo, Chenqi, Hu, Peike, Fu, Qiuli, Yao, Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291466/
https://www.ncbi.nlm.nih.gov/pubmed/37378332
http://dx.doi.org/10.1016/j.isci.2023.106921
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author Chen, Xinyi
Wang, Hanle
Chen, Hui
Ren, Ling
Wang, Wei
Xu, Jingjie
Luo, Chenqi
Hu, Peike
Fu, Qiuli
Yao, Ke
author_facet Chen, Xinyi
Wang, Hanle
Chen, Hui
Ren, Ling
Wang, Wei
Xu, Jingjie
Luo, Chenqi
Hu, Peike
Fu, Qiuli
Yao, Ke
author_sort Chen, Xinyi
collection PubMed
description Lens itself has limited regeneration functionality, thus we aimed to create regenerated lens with biological function to treat cataracts rather than the intraocular lens used in cataract surgery. We induced exogenous human embryonic stem cells to directionally differentiate into lens fate like cells in vitro, mixed these cells with hyaluronate, and then implanted the mixture into lens capsule to regenerate in vivo. We successfully achieved near-complete lens regeneration, and the thickness of the regenerated lens reached 85% of the contralateral eye, showing the characteristics of biconvex shape, transparency, and a thickness and diopter close to that of natural lenses. Meanwhile, the participation of Wnt/PCP pathway in lens regeneration was verified. The regenerated lens in this study was the most transparent, thickest, and most similar to the original natural lens that has thus far been reported. Overall, these findings offer a new therapeutic strategy for cataracts and other lens diseases.
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spelling pubmed-102914662023-06-27 Lens regeneration in situ using hESCs-derived cells —similar to natural lens Chen, Xinyi Wang, Hanle Chen, Hui Ren, Ling Wang, Wei Xu, Jingjie Luo, Chenqi Hu, Peike Fu, Qiuli Yao, Ke iScience Article Lens itself has limited regeneration functionality, thus we aimed to create regenerated lens with biological function to treat cataracts rather than the intraocular lens used in cataract surgery. We induced exogenous human embryonic stem cells to directionally differentiate into lens fate like cells in vitro, mixed these cells with hyaluronate, and then implanted the mixture into lens capsule to regenerate in vivo. We successfully achieved near-complete lens regeneration, and the thickness of the regenerated lens reached 85% of the contralateral eye, showing the characteristics of biconvex shape, transparency, and a thickness and diopter close to that of natural lenses. Meanwhile, the participation of Wnt/PCP pathway in lens regeneration was verified. The regenerated lens in this study was the most transparent, thickest, and most similar to the original natural lens that has thus far been reported. Overall, these findings offer a new therapeutic strategy for cataracts and other lens diseases. Elsevier 2023-05-19 /pmc/articles/PMC10291466/ /pubmed/37378332 http://dx.doi.org/10.1016/j.isci.2023.106921 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Chen, Xinyi
Wang, Hanle
Chen, Hui
Ren, Ling
Wang, Wei
Xu, Jingjie
Luo, Chenqi
Hu, Peike
Fu, Qiuli
Yao, Ke
Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title_full Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title_fullStr Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title_full_unstemmed Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title_short Lens regeneration in situ using hESCs-derived cells —similar to natural lens
title_sort lens regeneration in situ using hescs-derived cells —similar to natural lens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291466/
https://www.ncbi.nlm.nih.gov/pubmed/37378332
http://dx.doi.org/10.1016/j.isci.2023.106921
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