Exonuclease III Can Efficiently Cleave Linear Single-Stranded DNA: Reshaping Its Experimental Applications in Biosensors

Exonuclease III (Exo III) has been generally used as a double-stranded DNA (dsDNA)-specific exonuclease that does not degrade single-stranded DNA (ssDNA). Here, we demonstrate that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear ssDNA. Moreover, the dsDNA specificity of Exo...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Yi, Yuan, Haoyu, Guo, Zixuan, Li, Xiu-Qing, Yang, Zhiqing, Zong, Chengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295840/
https://www.ncbi.nlm.nih.gov/pubmed/37366946
http://dx.doi.org/10.3390/bios13060581

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295840/
https://www.ncbi.nlm.nih.gov/pubmed/37366946
http://dx.doi.org/10.3390/bios13060581

Ejemplares similares