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Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection

Coenzyme Q10 (CoQ10) is a vitamin-like compound found naturally in plant- and animal-derived materials. This study aimed to determine the level of CoQ10 in some food by-products (oil press cakes) and waste (fish meat and chicken hearts) to recover this compound for further use as a dietary supplemen...

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Autores principales: Semeniuc, Cristina Anamaria, Ranga, Floricuța, Podar, Andersina Simina, Ionescu, Simona Raluca, Socaciu, Maria-Ioana, Fogarasi, Melinda, Fărcaș, Anca Corina, Vodnar, Dan Cristian, Socaci, Sonia Ancuța
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297063/
https://www.ncbi.nlm.nih.gov/pubmed/37372507
http://dx.doi.org/10.3390/foods12122296
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author Semeniuc, Cristina Anamaria
Ranga, Floricuța
Podar, Andersina Simina
Ionescu, Simona Raluca
Socaciu, Maria-Ioana
Fogarasi, Melinda
Fărcaș, Anca Corina
Vodnar, Dan Cristian
Socaci, Sonia Ancuța
author_facet Semeniuc, Cristina Anamaria
Ranga, Floricuța
Podar, Andersina Simina
Ionescu, Simona Raluca
Socaciu, Maria-Ioana
Fogarasi, Melinda
Fărcaș, Anca Corina
Vodnar, Dan Cristian
Socaci, Sonia Ancuța
author_sort Semeniuc, Cristina Anamaria
collection PubMed
description Coenzyme Q10 (CoQ10) is a vitamin-like compound found naturally in plant- and animal-derived materials. This study aimed to determine the level of CoQ10 in some food by-products (oil press cakes) and waste (fish meat and chicken hearts) to recover this compound for further use as a dietary supplement. The analytical method involved ultrasonic extraction using 2-propanol, followed by high-performance liquid chromatography with diode array detection (HPLC-DAD). The HPLC-DAD method was validated in terms of linearity and measuring range, limits of detection (LOD) and quantification (LOQ), trueness, and precision. As a result, the calibration curve of CoQ10 was linear over the concentration range of 1–200 µg/mL, with an LOD of 22 µg/mL and an LOQ of 0.65 µg/mL. The CoQ10 content varied from not detected in the hempseed press cake and the fish meat to 84.80 µg/g in the pumpkin press cake and 383.25 µg/g in the lyophilized chicken hearts; very good recovery rates and relative standard deviations (RSDs) were obtained for the pumpkin press cake (100.9–116.0% with RSDs between 0.05–0.2%) and the chicken hearts (99.3–106.9% CH with RSDs between 0.5–0.7%), showing the analytical method’s trueness and precision and thus its accuracy. In conclusion, a simple and reliable method for determining CoQ10 levels has been developed here.
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spelling pubmed-102970632023-06-28 Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection Semeniuc, Cristina Anamaria Ranga, Floricuța Podar, Andersina Simina Ionescu, Simona Raluca Socaciu, Maria-Ioana Fogarasi, Melinda Fărcaș, Anca Corina Vodnar, Dan Cristian Socaci, Sonia Ancuța Foods Article Coenzyme Q10 (CoQ10) is a vitamin-like compound found naturally in plant- and animal-derived materials. This study aimed to determine the level of CoQ10 in some food by-products (oil press cakes) and waste (fish meat and chicken hearts) to recover this compound for further use as a dietary supplement. The analytical method involved ultrasonic extraction using 2-propanol, followed by high-performance liquid chromatography with diode array detection (HPLC-DAD). The HPLC-DAD method was validated in terms of linearity and measuring range, limits of detection (LOD) and quantification (LOQ), trueness, and precision. As a result, the calibration curve of CoQ10 was linear over the concentration range of 1–200 µg/mL, with an LOD of 22 µg/mL and an LOQ of 0.65 µg/mL. The CoQ10 content varied from not detected in the hempseed press cake and the fish meat to 84.80 µg/g in the pumpkin press cake and 383.25 µg/g in the lyophilized chicken hearts; very good recovery rates and relative standard deviations (RSDs) were obtained for the pumpkin press cake (100.9–116.0% with RSDs between 0.05–0.2%) and the chicken hearts (99.3–106.9% CH with RSDs between 0.5–0.7%), showing the analytical method’s trueness and precision and thus its accuracy. In conclusion, a simple and reliable method for determining CoQ10 levels has been developed here. MDPI 2023-06-07 /pmc/articles/PMC10297063/ /pubmed/37372507 http://dx.doi.org/10.3390/foods12122296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Semeniuc, Cristina Anamaria
Ranga, Floricuța
Podar, Andersina Simina
Ionescu, Simona Raluca
Socaciu, Maria-Ioana
Fogarasi, Melinda
Fărcaș, Anca Corina
Vodnar, Dan Cristian
Socaci, Sonia Ancuța
Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title_full Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title_fullStr Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title_full_unstemmed Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title_short Determination of Coenzyme Q10 Content in Food By-Products and Waste by High-Performance Liquid Chromatography Coupled with Diode Array Detection
title_sort determination of coenzyme q10 content in food by-products and waste by high-performance liquid chromatography coupled with diode array detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297063/
https://www.ncbi.nlm.nih.gov/pubmed/37372507
http://dx.doi.org/10.3390/foods12122296
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