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Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression
Proteolytic activity is pivotal in maintaining cell homeostasis and function. In pathological conditions such as cancer, it covers a key role in tumor cell viability, spreading to distant organs, and response to the treatment. Endosomes represent one of the major sites of cellular proteolytic activi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299664/ https://www.ncbi.nlm.nih.gov/pubmed/37373389 http://dx.doi.org/10.3390/ijms241210245 |
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author | Kolesova, Ekaterina P. Egorova, Vera S. Syrocheva, Anastasiia O. Frolova, Anastasiia S. Kostyushev, Dmitry Kostyusheva, Anastasiia Brezgin, Sergey Trushina, Daria B. Fatkhutdinova, Landysh Zyuzin, Mikhail Demina, Polina A. Khaydukov, Evgeny V. Zamyatnin, Andrey A. Parodi, Alessandro |
author_facet | Kolesova, Ekaterina P. Egorova, Vera S. Syrocheva, Anastasiia O. Frolova, Anastasiia S. Kostyushev, Dmitry Kostyusheva, Anastasiia Brezgin, Sergey Trushina, Daria B. Fatkhutdinova, Landysh Zyuzin, Mikhail Demina, Polina A. Khaydukov, Evgeny V. Zamyatnin, Andrey A. Parodi, Alessandro |
author_sort | Kolesova, Ekaterina P. |
collection | PubMed |
description | Proteolytic activity is pivotal in maintaining cell homeostasis and function. In pathological conditions such as cancer, it covers a key role in tumor cell viability, spreading to distant organs, and response to the treatment. Endosomes represent one of the major sites of cellular proteolytic activity and very often represent the final destination of internalized nanoformulations. However, little information about nanoparticle impact on the biology of these organelles is available even though they represent the major location of drug release. In this work, we generated albumin nanoparticles with a different resistance to proteolysis by finely tuning the amount of cross-linker used to stabilize the carriers. After careful characterization of the particles and measurement of their degradation in proteolytic conditions, we determined a relationship between their sensitivity to proteases and their drug delivery properties. These phenomena were characterized by an overall increase in the expression of cathepsin proteases regardless of the different sensitivity of the particles to proteolytic degradation. |
format | Online Article Text |
id | pubmed-10299664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102996642023-06-28 Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression Kolesova, Ekaterina P. Egorova, Vera S. Syrocheva, Anastasiia O. Frolova, Anastasiia S. Kostyushev, Dmitry Kostyusheva, Anastasiia Brezgin, Sergey Trushina, Daria B. Fatkhutdinova, Landysh Zyuzin, Mikhail Demina, Polina A. Khaydukov, Evgeny V. Zamyatnin, Andrey A. Parodi, Alessandro Int J Mol Sci Article Proteolytic activity is pivotal in maintaining cell homeostasis and function. In pathological conditions such as cancer, it covers a key role in tumor cell viability, spreading to distant organs, and response to the treatment. Endosomes represent one of the major sites of cellular proteolytic activity and very often represent the final destination of internalized nanoformulations. However, little information about nanoparticle impact on the biology of these organelles is available even though they represent the major location of drug release. In this work, we generated albumin nanoparticles with a different resistance to proteolysis by finely tuning the amount of cross-linker used to stabilize the carriers. After careful characterization of the particles and measurement of their degradation in proteolytic conditions, we determined a relationship between their sensitivity to proteases and their drug delivery properties. These phenomena were characterized by an overall increase in the expression of cathepsin proteases regardless of the different sensitivity of the particles to proteolytic degradation. MDPI 2023-06-16 /pmc/articles/PMC10299664/ /pubmed/37373389 http://dx.doi.org/10.3390/ijms241210245 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kolesova, Ekaterina P. Egorova, Vera S. Syrocheva, Anastasiia O. Frolova, Anastasiia S. Kostyushev, Dmitry Kostyusheva, Anastasiia Brezgin, Sergey Trushina, Daria B. Fatkhutdinova, Landysh Zyuzin, Mikhail Demina, Polina A. Khaydukov, Evgeny V. Zamyatnin, Andrey A. Parodi, Alessandro Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title | Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title_full | Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title_fullStr | Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title_full_unstemmed | Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title_short | Proteolytic Resistance Determines Albumin Nanoparticle Drug Delivery Properties and Increases Cathepsin B, D, and G Expression |
title_sort | proteolytic resistance determines albumin nanoparticle drug delivery properties and increases cathepsin b, d, and g expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299664/ https://www.ncbi.nlm.nih.gov/pubmed/37373389 http://dx.doi.org/10.3390/ijms241210245 |
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