Long-Read Nanopore Sequencing of RPGR ORF15 is Enhanced Following DNase I Treatment of MinION Flow Cells

INTRODUCTION: RPGR ORF15 is an exon present almost exclusively in the retinal transcript of RPGR. It is purine-rich, repetitive and notoriously hard to sequence, but is a hotspot for mutations causing X-linked retinitis pigmentosa. METHODS: Long-read nanopore sequencing on MinION and Flongle flow ce...

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Detalles Bibliográficos
Autores principales: Yahya, Samar, Watson, Christopher M., Carr, Ian, McKibbin, Martin, Crinnion, Laura A., Taylor, Morag, Bonin, Hope, Fletcher, Tracy, El-Asrag, Mohammed E., Ali, Manir, Toomes, Carmel, Inglehearn, Chris F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299921/
https://www.ncbi.nlm.nih.gov/pubmed/37284979
http://dx.doi.org/10.1007/s40291-023-00656-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299921/
https://www.ncbi.nlm.nih.gov/pubmed/37284979
http://dx.doi.org/10.1007/s40291-023-00656-z

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