Differential affinity purification and mass spectrometry analysis of two nuclear pore complex isoforms in yeast S. cerevisiae

Two isoforms of the nuclear pore complex (NPC) have been identified in the yeast S. cerevisiae, which coexist at the periphery of the nucleus and differ by the presence or absence of a nuclear basket. Here, we present a protocol to isolate the two types of NPCs from the same cell extract and dissect...

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Detalles Bibliográficos
Autores principales: Bensidoun, Pierre, Zenklusen, Daniel, Oeffinger, Marlene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300402/
https://www.ncbi.nlm.nih.gov/pubmed/37327111
http://dx.doi.org/10.1016/j.xpro.2023.102359

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300402/
https://www.ncbi.nlm.nih.gov/pubmed/37327111
http://dx.doi.org/10.1016/j.xpro.2023.102359

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