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Differential affinity purification and mass spectrometry analysis of two nuclear pore complex isoforms in yeast S. cerevisiae
Two isoforms of the nuclear pore complex (NPC) have been identified in the yeast S. cerevisiae, which coexist at the periphery of the nucleus and differ by the presence or absence of a nuclear basket. Here, we present a protocol to isolate the two types of NPCs from the same cell extract and dissect...
Autores principales: | Bensidoun, Pierre, Zenklusen, Daniel, Oeffinger, Marlene |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300402/ https://www.ncbi.nlm.nih.gov/pubmed/37327111 http://dx.doi.org/10.1016/j.xpro.2023.102359 |
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