Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 diversely enhances selection efficiency of CHO cells expressing therapeutic antibodies

The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, for which GS-knockout (GS-KO) CHO cell lines are commonly used. Since genome analysis indi...

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Detalles Bibliográficos
Autores principales: Srila, Witsanu, Baumann, Martina, Riedl, Markus, Rangnoi, Kuntalee, Borth, Nicole, Yamabhai, Montarop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307828/
https://www.ncbi.nlm.nih.gov/pubmed/37380701
http://dx.doi.org/10.1038/s41598-023-37288-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307828/
https://www.ncbi.nlm.nih.gov/pubmed/37380701
http://dx.doi.org/10.1038/s41598-023-37288-6

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