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β-lactamase (Bla) Reporter-based System to Study Flagellar Type 3 Secretion in Salmonella
Export of type 3 secretion (T3S) substrates is traditionally evaluated using trichloroacetic acid (TCA) precipitation of cultured cell supernatants followed by western blot analysis of the secreted substrates. In our lab, we have developed β-lactamase (Bla), lacking its Sec secretion signal, as a re...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Bio-Protocol
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308186/ https://www.ncbi.nlm.nih.gov/pubmed/37397791 http://dx.doi.org/10.21769/BioProtoc.4696 |