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β-lactamase (Bla) Reporter-based System to Study Flagellar Type 3 Secretion in Salmonella

Export of type 3 secretion (T3S) substrates is traditionally evaluated using trichloroacetic acid (TCA) precipitation of cultured cell supernatants followed by western blot analysis of the secreted substrates. In our lab, we have developed β-lactamase (Bla), lacking its Sec secretion signal, as a re...

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Detalles Bibliográficos
Autores principales:	Chevance, Fabienne F. V., Hughes, Kelly T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bio-Protocol 2023
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308186/ https://www.ncbi.nlm.nih.gov/pubmed/37397791 http://dx.doi.org/10.21769/BioProtoc.4696

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