Purification and characterization of inorganic pyrophosphatase for in vitro RNA transcription

Inorganic pyrophosphatase (iPPase) is an enzyme that cleaves pyrophosphate into two phosphate molecules. This enzyme is an essential component of in vitro transcription (IVT) reactions for RNA preparation as it prevents pyrophosphate from precipitating with magnesium, ultimately increasing the rate...

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Detalles Bibliográficos
Autores principales: Tersteeg, Scott, Mrozowich, Tyler, Henrickson, Amy, Demeler, Borries, Patel, Trushar R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311840/
https://www.ncbi.nlm.nih.gov/pubmed/35926232
http://dx.doi.org/10.1139/bcb-2022-0118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10311840/
https://www.ncbi.nlm.nih.gov/pubmed/35926232
http://dx.doi.org/10.1139/bcb-2022-0118

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