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Enhancing single-cell western blotting sensitivity using diffusive analyte blotting and antibody conjugate amplification

While there are many techniques to achieve highly sensitive, multiplex detection of RNA and DNA from single cells, detecting protein contents often suffers from low limits of detection and throughput. Miniaturized, high-sensitivity western blots on single cells (scWesterns) are attractive since they...

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Detalles Bibliográficos
Autores principales: Long, Mariia Alibekova, Benman, William, Petrikas, Nathan, Bugaj, Lukasz J., Hughes, Alex J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312704/
https://www.ncbi.nlm.nih.gov/pubmed/37398364
http://dx.doi.org/10.1101/2023.06.13.544857