Formalin fixation and paraffin embedding interfere with preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two photon excited fluorescence

Endogenous NAD(P)H and FAD two-photon excited fluorescence (TPEF) images provide functional metabolic information with high spatial resolution for a wide range of living specimens. Preservation of metabolic function optical metrics upon fixation would facilitate studies which assess the impact of me...

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Detalles Bibliográficos
Autores principales: Sánchez-Hernández, Adriana, Polleys, Christopher M., Georgakoudi, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312786/
https://www.ncbi.nlm.nih.gov/pubmed/37398103
http://dx.doi.org/10.1101/2023.06.16.545363

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312786/
https://www.ncbi.nlm.nih.gov/pubmed/37398103
http://dx.doi.org/10.1101/2023.06.16.545363

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