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Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle

BACKGROUND: Intramuscular fat (IMF) is closely related to the tenderness, marbling, juiciness, and flavor of meat. We used a combined transcriptome and metabolome analysis to investigate the molecular mechanisms underlying phenotypic variation among Qinchuan cattle. RESULTS: The IMF content was rela...

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Autores principales: Yu, Hengwei, Wang, Jianfang, Zhang, Ke, Cheng, Gong, Mei, Chugang, Zan, Linsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314489/
https://www.ncbi.nlm.nih.gov/pubmed/37391702
http://dx.doi.org/10.1186/s12864-023-09452-9
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author Yu, Hengwei
Wang, Jianfang
Zhang, Ke
Cheng, Gong
Mei, Chugang
Zan, Linsen
author_facet Yu, Hengwei
Wang, Jianfang
Zhang, Ke
Cheng, Gong
Mei, Chugang
Zan, Linsen
author_sort Yu, Hengwei
collection PubMed
description BACKGROUND: Intramuscular fat (IMF) is closely related to the tenderness, marbling, juiciness, and flavor of meat. We used a combined transcriptome and metabolome analysis to investigate the molecular mechanisms underlying phenotypic variation among Qinchuan cattle. RESULTS: The IMF content was relatively high in the meat of Qinchuan cattle bulls and differed among muscle locations, namely the high rib (15.86%), ribeye (14%), striploin (10.44%), and tenderloin (8.67%). CCDC80 and the HOX gene cluster may regulate intramuscular adipose tissue deposition. Moreover, erucic acid (EA) was found to be the main metabolite in Qinchuan beef cattle, with a high concentration in IMF. The deposition of IMF could be regulated by the metabolic pathway for unsaturated fatty acids involving EA and the ACOX3, HACD2, and SCD5 genes. In addition, differentially expressed genes and metabolites were enriched in three major KEGG pathways: purine metabolism, pyrimidine metabolism, and the metabolism of glycine, serine, and threonine. CONCLUSIONS: We identified a significant metabolite, EA, with variation in IMF. Its closely related genes, ACOX3, HACD2, and SCD5, co-regulate the metabolism of unsaturated fatty acids, ultimately affecting the accumulation of intramuscular adipose tissue in Qinchuan cattle. Consequently, Qinchuan cattle are an elite cultivar for high-quality beef production and have great potential for breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09452-9.
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spelling pubmed-103144892023-07-02 Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle Yu, Hengwei Wang, Jianfang Zhang, Ke Cheng, Gong Mei, Chugang Zan, Linsen BMC Genomics Research BACKGROUND: Intramuscular fat (IMF) is closely related to the tenderness, marbling, juiciness, and flavor of meat. We used a combined transcriptome and metabolome analysis to investigate the molecular mechanisms underlying phenotypic variation among Qinchuan cattle. RESULTS: The IMF content was relatively high in the meat of Qinchuan cattle bulls and differed among muscle locations, namely the high rib (15.86%), ribeye (14%), striploin (10.44%), and tenderloin (8.67%). CCDC80 and the HOX gene cluster may regulate intramuscular adipose tissue deposition. Moreover, erucic acid (EA) was found to be the main metabolite in Qinchuan beef cattle, with a high concentration in IMF. The deposition of IMF could be regulated by the metabolic pathway for unsaturated fatty acids involving EA and the ACOX3, HACD2, and SCD5 genes. In addition, differentially expressed genes and metabolites were enriched in three major KEGG pathways: purine metabolism, pyrimidine metabolism, and the metabolism of glycine, serine, and threonine. CONCLUSIONS: We identified a significant metabolite, EA, with variation in IMF. Its closely related genes, ACOX3, HACD2, and SCD5, co-regulate the metabolism of unsaturated fatty acids, ultimately affecting the accumulation of intramuscular adipose tissue in Qinchuan cattle. Consequently, Qinchuan cattle are an elite cultivar for high-quality beef production and have great potential for breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09452-9. BioMed Central 2023-07-01 /pmc/articles/PMC10314489/ /pubmed/37391702 http://dx.doi.org/10.1186/s12864-023-09452-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yu, Hengwei
Wang, Jianfang
Zhang, Ke
Cheng, Gong
Mei, Chugang
Zan, Linsen
Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title_full Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title_fullStr Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title_full_unstemmed Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title_short Integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of Qinchuan cattle
title_sort integrated multi-omics analysis reveals variation in intramuscular fat among muscle locations of qinchuan cattle
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314489/
https://www.ncbi.nlm.nih.gov/pubmed/37391702
http://dx.doi.org/10.1186/s12864-023-09452-9
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