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Genome-scale targeted mutagenesis in Brassica napus using a pooled CRISPR library

The recently constructed mutant libraries of diploid crops by the CRISPR-Cas9 system have provided abundant resources for functional genomics and crop breeding. However, because of the genome complexity, it is a big challenge to accomplish large-scale targeted mutagenesis in polyploid plants. Here,...

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Detalles Bibliográficos
Autores principales: He, Jianjie, Zhang, Kai, Yan, Shuxiang, Tang, Mi, Zhou, Weixian, Yin, Yongtai, Chen, Kang, Zhang, Chunyu, Li, Maoteng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10317123/
https://www.ncbi.nlm.nih.gov/pubmed/37290935
http://dx.doi.org/10.1101/gr.277650.123