A rapid cell-free expression and screening platform for antibody discovery

Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this bottleneck by developing a workflow combining cell-free DNA template generation, cell-free protein synthesis, and binding measurements of antibody fragments in a process tha...

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Detalles Bibliográficos
Autores principales: Hunt, Andrew C., Vögeli, Bastian, Hassan, Ahmed O., Guerrero, Laura, Kightlinger, Weston, Yoesep, Danielle J., Krüger, Antje, DeWinter, Madison, Diamond, Michael S., Karim, Ashty S., Jewett, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318062/
https://www.ncbi.nlm.nih.gov/pubmed/37400446
http://dx.doi.org/10.1038/s41467-023-38965-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318062/
https://www.ncbi.nlm.nih.gov/pubmed/37400446
http://dx.doi.org/10.1038/s41467-023-38965-w

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