High-efficiency targeted transgene integration via primed micro-homologues

Due to the difficulties in precisely manipulating DNA repair pathways, high-fidelity targeted integration of large transgenes triggered by double-strand breaks is inherently inefficient. Here, we exploit prime editors to devise a robust knock-in (KI) strategy named primed micro-homologues-assisted i...

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Detalles Bibliográficos
Autores principales: Wang, Chenxin, Fang, Sen, Chen, Yangcan, Tang, Na, Jiao, Guanyi, Hu, Yanping, Li, Jing, Shan, Qingtong, Wang, Xin, Feng, Guihai, Zhou, Qi, Li, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319781/
https://www.ncbi.nlm.nih.gov/pubmed/37402729
http://dx.doi.org/10.1038/s41421-023-00552-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10319781/
https://www.ncbi.nlm.nih.gov/pubmed/37402729
http://dx.doi.org/10.1038/s41421-023-00552-0

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