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A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro

INTRODUCTION: Glass coverslips are used as a substrate since Harrison’s initial nerve cell culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine (PL) coated substrate was published. Usually, neurons adhere quickly to PL coating. However, maintaining cortical neu...

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Detalles Bibliográficos
Autores principales: Stil, Aurélie, Liberelle, Benoît, Guadarrama Bello, Dainelys, Lacomme, Lucile, Arpin, Laurie, Parent, Pascale, Nanci, Antonio, Dumont, Éric C., Ould-Bachir, Tarek, Vanni, Matthieu P., De Crescenzo, Gregory, Bouchard, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320290/
https://www.ncbi.nlm.nih.gov/pubmed/37416506
http://dx.doi.org/10.3389/fncel.2023.1212097