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A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro
INTRODUCTION: Glass coverslips are used as a substrate since Harrison’s initial nerve cell culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine (PL) coated substrate was published. Usually, neurons adhere quickly to PL coating. However, maintaining cortical neu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320290/ https://www.ncbi.nlm.nih.gov/pubmed/37416506 http://dx.doi.org/10.3389/fncel.2023.1212097 |