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ATAC-seq protocol for the profiling of chromatin accessibility of in situ fixed quiescent and activated muscle stem cells

Chromatin accessibility is critical for cell identity. Conventional ATAC-seq can examine chromatin accessibility on freshly prepared muscle stem cells or satellite cells (SCs); however, isolating SCs in mice remains challenging. Here, we present a protocol to preserve the in vivo chromatin profile o...

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Detalles Bibliográficos
Autores principales: Dong, Anqi, Chan, Indigo T.C., Cheung, Tom H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10320306/
https://www.ncbi.nlm.nih.gov/pubmed/37352103
http://dx.doi.org/10.1016/j.xpro.2023.102376